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. 2007 Jan 22;25(18):3599–3605. doi: 10.1016/j.vaccine.2007.01.055

Fig. 4.

Fig. 4

(a) Amount of IFN-γ released from primed cells by ex vivo FliC-restimulation (n = 3). Spleen cells from immunized mice were incubated with or without FliC and the amount of IFN-γ released was measured by ELISA. Values are given as means + S.D. (b) Intracellular cytokine (IFN-γ) staining of primed cells (n = 3). Ten thousand events were acquired and the percentage of CD4+ cells containing IFN-γ was analyzed. The figures represent one mouse of each group, and the values are given as the mean ± S.D.