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. 2018 Feb 1;36(8):1085–1092. doi: 10.1016/j.vaccine.2018.01.017

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© 2018 The Authors

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 2 — Immunohistochemistry of CAMs after rescue of rIBV-Δ3ab, rIBV-Δ5ab and rIBV-Δ3ab5ab. Embryonated chicken eggs were inoculated with mIBV-infected LR7 cells that had been transfected with transcripts from donor plasmids p-IBV-Δ3ab, -Δ5ab or -Δ3ab5ab by electroporation. mIBV-infected non-transfected LR7 cells served as controls. Formalin-fixed and paraffin-embedded CAMs were stained using a monoclonal antibody against IBV-S2.