Table 1.

Plasmids used for generation of the donor plasmids p-IBV-Δ3ab, -Δ5ab and -Δ3ab5ab.

Plasmid	Genes	Coordinates	Length (nt)	3′-end RES	Surrounding RES	Inserted in plasmid
p-IBV-5	5′-UTR	1…497	497	BstBI	n.a.	n.a.
p-IBV-1b	1b, S	19,610…20,379	770	XhoI	BstBI	p-IBV-5
p-IBV-S	S	20,379…23,590	3211	StyI	XhoI	p-IBV-5-1b
p-IBV-SIR	S, 3ab, E, M	23,591…25,318	1728	EcoRI	StyI	p-IBV-5-1b-S
p-IBV-3 T	5ab, N, 3′-UTR	25,319…27,730	2422	MssI, PacI	EcoRI	p-IBV-5-1b-S-SIR
p-Δ3ab	n.a.	23,604…24,304	349	n.a.	NheI, PmlI	p-IBV-5-1b-S-SIR
p-Δ5ab	n.a.	25,467…25,974	115	n.a.	AfeI, NheI	p-IBV-3 T