Table 2.
Primer sets used for the characterization of the 3′ 9 kb of the viral genome of rIBVs.
| Primer set | Target | Primer | Sequence (5′ → 3′) | Amplicon (bp)a |
|---|---|---|---|---|
| C | IBV 1b – IBV S | IBV.F73 | TCAGCATGGACGTGTGGTTA | 992 |
| IBV.R73 | CCCCATGTAAATGCCAACCA | |||
| E | IBV S – IBV M | IBV.F15 | TGCTGCTTCCTTTAATAAG | 1994/1646 |
| IBV.R15 | CTGCGACAAGACCTCCTG | |||
| I | IBV IR – IBV N | IBV.F28 | TGTTGTAGGTTGTGGTCCCA | 1633/1248 |
| IBV.R16 | CTGAGGTCAATGCCTTATC | |||
a
For primer sets [E] and [I], the amplicon lengths for rIBV with/without gene 3 and/or 5 are given.