Table 2.

Summary of percentage CD8⁺ IFNγ⁺ responses in SARS subject 1 induced by truncated peptides within M29 region. T cells used were obtained from SARS subject 1 at 9 years post-infection. Results of positive peptides (M29, M29_144–155, M29_145–155, M29_146–155, M29_147–155) and selected negative peptides (M29_143–154, M29_145–154, M29_148–155) are shown. Percentage CD8⁺IFNγ⁺ T cells shown represents the percentage of IFNγ-producing CD8⁺ T cells in the total T cell population (after gating the CD3⁺ cells) present in the short-term T cell line obtained by restimulation using M29 peptide. The minimal epitope is indicated in italics.

Peptide	Peptide length	Amino acid position	Peptide sequence	Percentage of CD8+IFNγ+ T cells
M29	15-mer	141–155	AVIIR GHLRM AGHSL	5.3%
M29144–155	12-mer	144–155	IR GHLRM AGHSL	14.8%
M29143–154	12-mer	143–154	IIR GHLRM AGHS	0.2%
M29145–155	11-mer	145–155	R GHLRM AGHSL	17.8%
M29146–155	10-mer	146–155	GHLRM AGHSL	22.8%
M29145–154	10-mer	145–154	R GHLRM AGHS	0.2%
M29147–155	9-mer	147–155	HLRM AGHSL	32.9%
M29148–155	8-mer	148–155	LRM AGHSL	0.3%
Unstimulated cells				0.2%
PMA/Ionomycin-stimulated cells				11.1%