Table 3.
Summary of percentage CD8+IFNγ+ responses in SARS subject 1 induced by truncated peptides within N53 region. T cells used were obtained from SARS subject 1 at 6 years post-infection. Percentage of CD8+IFNγ+ cells shown represent the percentage of IFNγ-producing CD8+ cells in the total T cell population (after gating the CD3+ cells) present in the short-term T cell line obtained from restimulation using N53 peptide. The minimal epitope is indicated in italics.
| Peptide | Peptide length | Amino acid position | Peptide sequence | Percentage of CD8+IFNγ+ T cells |
|---|---|---|---|---|
| N53 | 15-mer | 261–275 | QKRTA TKQYN VTQAF | 8.8% |
| N53266–275 | 10-mer | 266–275 | TKQYN VTQAF | 12.7% |
| N53266–274 | 9-mer | 266–274 | TKQYN VTQA | 8.0% |
| N53267–275 | 9-mer | 267–275 | KQYN VTQAF | 10.9% |
| N53266–273 | 8-mer | 266–273 | TKQYN VTQ | 2.2% |
| N53267–274 | 8-mer | 267–274 | KQYN VTQA | 2.3% |
| N53268–275 | 8-mer | 268–275 | QYN VTQAF | 2.2% |
| Unstimulated cells | 1.6% | |||
| PMA/Ionomycin-stimulated cells | 15.3% |