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The viral loads in the lungs were examined by conducting quantitative real-time RT-PCR on total RNA extracted from homogenates of pieces from the different pulmonary lobes
| Experimental group | Calf | BRSV RNA copies/106 ACTB RNA copies |
|---|---|---|
| Mock | 6601 | 5.5 × 103 |
| 6604 | 2.0 × 104 | |
| 6621 | 5.2 × 105 | |
| 6628 | 1.2 × 103 | |
| Npur | 6615 | – |
| 6622 | 6.8 × 104 | |
| 6626 | – | |
| pNsyn | 6596 | – |
| 6610 | – | |
| 6624 | – | |
| pNsyn–Npur | 6603 | – |
| 6611 | – | |
| 6612 | – | |
| 6620 | – | |
(–) Not detected.
