Fig. 6.

Exposure of Murine BMDC to ALVAC enhances CXCL10 and CCL2 secretion. Murine bone-marrow derived DCs were incubated for 24 h with either medium alone, Placebo (equivalent volume to ALVAC) both to serve as negative controls; ALVAC at an MOI of 0.2 or a TLR4 agonist at a concentration of 0.1 μg/ml. Supernatant was removed and analysed for CXCL10 (A), CCL2 (B), CXCL16 (C), IL-6 (D), TNF-α (E), and IL-12p40 (F) by ELISA. Results are expressed as mean concentration ± standard deviation and are representative of three independent experiments.