Fig. 4.

Neutralizing antibody titers in Balb/c mice after immunization using WNVwt and WNVsyn derived vaccine preparations (a) and characterization of the inactivated vaccine preparations used to immunize mice by Western blotting (b). Gray bars represent neutralizing antibody titers of mice vaccinated with WNVwt antigen and white bars neutralizing antibody titers of mice vaccinated with WNVsyn preparations (upper panel). Western blotting was carried out to analyze the antigen content used in individual formulations (lower panel); w, WNVwt; s, WNVsyn; E, WNV envelope protein; prM, pre-membrane protein; M*, dimeric membrane protein.