Fig. 8.

BRSV-specific lymphocyte proliferative responses in vaccinated calves. Calves (identified as calf ‘a’ through ‘t’) were immunized as described in Fig. 1 and challenged with BRSV on PID 0. Peripheral blood mononuclear cells were obtained on PID −3 or −2 (before challenge, dark grey bars) and PID 4 or 5 (after challenge, light grey bars), and were stimulated with BRSV or control antigen. Proliferation is expressed as corrected OD values (mean of triplicate) after six days of stimulation and addition of Alamar Blue^® reagent.