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Stepwise mass balance of HA-NC purification by SRID assay
| HA (μg/ml) | Volume (ml) | HA (mg) | Puritya (%) | Step recovery (%) | Total recovery (%) | |
|---|---|---|---|---|---|---|
| Starting material | 53 | 225 | 12 | 3.6 | ||
| Q/SP | 240 | 40 | 9.6 | 52 | 80 | 80 |
| HX-1 | 280 | 25 | 7.0 | 91 | 73 | 58 |
| Ultrafiltration | 700 | 9.1 | 6.4 | 99 | 91 | 53 |
Purity was determined by the densitometry of SDS–PAGE.
