Table 1.
Examples for diagnostic real-time PCR assays developed at the OIE Collaborating Centre (SVA, Uppsala)
| Assay | Targeted region |
|---|---|
| Equine herpesvirus 1 | Glycoprotein C |
| Equine herpesvirus 4 | Thymidine kinase |
| Equine influenza A | Non-structural protein (NS1) |
| Equine rhinovirus B | Polyprotein |
| Parvovirus, canine/feline | VP2 |
| Feline leukaemia virus | Envelope protein |
| Bovine respiratory syncytial virus | Fusion protein (F) |
| Bovine coronavirus | Haemagglutinin-esterase (HE) |
| Bovine rotavirus | VP6 |
| Bovine parainfluenza 3 virus | Fusion protein (F) |
| Bovine viral diarrhoea virus | 5′-non coding region |
| Swine vesicular disease virus | Polymerase protein (3D) |
| Deformed wing virus, honey bee | Polyprotein |
| Mouse hepatitis virus | E1 (M) protein |
| Lactate dehydrogenase elevating virus | ORF 7 |
| Mink astrovirus | RNA polymerase |
These systems are regularly used in routine diagnostic work [26, Belák and Thorén, OIE Collaborating Centre Report 2005].