Table 2.

Immune response and protection of CD1 mice

Dose (μg)	Animal	Non-adjuvanted			Adjuvanted with 0.05% aluminium hydroxide
		ELISA titre (week 4)	NT (week 5)	Virus titre (day 3 p.i.)	ELISA titre (week 4)	NT (week 5)	Virus titre (day 3 p.i.)
0.2	1	1:102,400	1:1522	≤1.7	1:25,600	1:1522	≤1.7
	2	1:25,600	1:3620	≤1.7	1:25,600	1:1256	≤1.6
	3	1:25,600	1:1396	≤1.6	1:102,400	1:1974	≤1.5
	4	1:25,600	1:830	≤1.6	1:25,600	1:1810	≤1.7
0.04	1	1:25,600	1:905	≤1.7	1:25,600	1:415	≤1.6
	2	1:25,600	1:190	≤1.6	1:400	≤1:28	≤1.6
	3	1:6400	1:587	≤1.6	1:6400	1:349	≤1.6
	4	1:6400	1:95	≤1.7	<1:100	≤1:33	2.2
0.008	1	1:1600	1:113	≤1.5	1:1600	1:37	≤1.6
	2	1:6400	1:320	≤1.7	1:1600	≤1:37	≤1.6
	3	1:25,600	1:415	≤1.6	1:25,600	1:587	≤1.6
	4	1:400	1:34	2.3	1:25,600	1:453	≤1.6
0.0016	1	1:400	≤1:57	3.4	1:1600	1:160	≤1.5
	2	1:1600	≤1:57	≤1.5	1:400	≤1:28	≤1.5
	3	<1:100	≤1:57	≤1.6	1:1600	≤1:28	≤1.5
	4	1:1600	≤1:57	2.7	1:400	≤1:28	2.3
0.0003	1	1:100	≤1:71	≤1.6	1:100	≤1:57	4.8
	2	<1:100	≤1:57	1.9	<1:100	≤1:57	3.6
	3	<1:100	≤1:71	5.6	1:100	≤1:57	3.4
	4	1:100	≤1:57	6.0	1:400	≤1:57	≤1.5
None	1	<1:100 (pool)	≤1:57	4.5
	2		≤1:57	4.8
	3		≤1:28	5.1
	4		≤1:57	3.4

p.i.: post inoculation. S-specific antibody titres were measured by ELISA, neutralising antibody titres (NT) by a micro neutralisation assay and SARS-CoV titres of homogenised lung/trachea tissue were determined by a TCID₅₀ assay and are given as log(TCID₅₀/g lung tissue).