Fig. 3.

Humoral immune response in mice vaccinated with various RV-G plasmid DNA constructs. Female BALB/c mice (four- to six-week-old) were immunized i.m. with plasmid DNA constructs encoding RV-G (100 μg/mice), vector (100 μg/mice) or PBS on days 0, 21 and 42. On days 20, 41 and 62, mice were bled and the sera were prepared; and subsequently analyzed for anti-RV-G antibodies by ELISA. Microtitration plates were coated with bacterially expressed, recombinant glycoprotein (500 ng/well) and incubated with 1:50 dilutions of immune sera samples. ELISA antibody titers are presented as the mean from all mice in each group.