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Expression of mRNA of (a) IL-2, (b) IFNγ and (c) IL-4 in Con A stimulated, C. felis-antigen stimulated and unstimulated PBMC cultures. The cytokine expression was normalised to GAPDH, which was set to a theoretical value of 1,000,000 gene copies. Statistical analysis was performed using Kruskal–Wallis and Dunn's multiple comparison test (*, P < 0.05; **, P < 0.01; ***, P < 0.005; ns = not significantly different).
