Table 12.
Cytotoxicity and antiviral activity of synthesized benzimidazole derivatives in HeLa cell cultures.
| Comp. | Cytotoxicity (μg/ml) |
EC50c (μg/ml) |
||||||
|---|---|---|---|---|---|---|---|---|
| CC50a | Minimum cytotoxic concentrationb | Vesicular stomatitis virus |
Coxsackie virus B4 |
Respiratory syncytial virus |
||||
| visual CPE score | MTS | visual CPE score | MTS | visual CPE score | MTS | |||
| 1 | >100 | ≥20 | >20 | >20 | >20 | >20 | >20 | >20 |
| 2 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 3 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 4 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 5 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 6 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 7 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 8 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 9 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 10 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 11 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 12 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 13 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 14 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 15 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 16 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 17 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 18 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 19 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| 20 | >100 | >100 | >100 | >100 | >100 | >100 | >100 | >100 |
| DS-5000 | >100 | >100 | >100 | >100 | 20 | 80.5 | 4 | 3.6 |
| (S)-DHPA (μM) | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 |
| Ribavirin (μM) | >250 | >250 | 10 | 3.6 | 50 | 14 | 6 | 2.8 |
a
50% Cytotoxic concentration, as determined by measuring the cell viability with the colorimetric formazan-based MTS assay.
b
Minimum compound concentration that causes a microscopically detectable alteration of normal cell morphology.
c
50% Effective concentration, or concentration resulting in 50% inhibition of virus-induced cytopathic effect, as determined by visual scoring of the CPE, or by measuring the cell viability with the colorimetric formazan-based MTS assay.