Table 1.
Vaccines tested in parallel in bovines and guinea pigs included in the concordance analysis.
| Type of vaccine | Syndrome | Vaccine composition viral antigens | BoHV-1 concentration (TCID50/dose) | Number of vaccines tested [1] | Number of vaccinated bovines | Number of vaccinated guinea pigs | Number of comparative assaysc |
|---|---|---|---|---|---|---|---|
| Calibration vaccines for the dose–response curve a (11 vaccines; 20 comparative assays) d | Respiratory | IBR-BVDV-PI-3 | 1 × 108 | 2 | 20 | 20 | 4 |
| 1 × 107 | 3 | 30 | 30 | 6 | |||
| 1 × 106 | 3 | 30 | 36 | 6 | |||
| 1 × 105 | 3 | 30 | 30 | 4 | |||
| Reference vaccines for concordance analysisb (n = 7 vaccines; 12 assays)d | Reproductive | IBR-BVDV | 107 | 3 | 70 | 51 | 8 |
| DIVA gE- | 107 | 1 | 5 | 5 | 1 | ||
| Respiratory | IBR-BVDV-PI-3 | 108 | 1 | 5 | 5 | 1 | |
| 107.5 | 1 | 5 | 5 | 1 | |||
| 107 | 1 | 5 | 5 | 1 | |||
| Commercial vaccinesc (n = 18 vaccines; 22 assays)d | Respiratory | IBR-BVDV-PI-3-BRSV | Unknown | 3 | 30 | 30 | 5 |
| IBR-BVDV-PI-3 | 5 | 29 | 40 | 6 | |||
| Reproductive | IBR-BVDV | 4 | 35 | 19 | 4 | ||
| Conjunctivitis | IBR | 2 | 15 | 11 | 2 | ||
| Multi-purpose | IBR-BVDV-PI-3-RV | 4 | 35 | 35 | 5 | ||
| Placebo | 8 | 58 | 44 | 8 | |||
| Total vaccinated | 44 | 402 | 366 | 62 | |||
| Non-vaccinated | 151 | 131 | 23 | ||||
| Total | 44 | 553 | 497 | 85 | |||
Three sets of water-in-oil vaccines with decreasing concentrations of BEI-inactivated BoHV-1 emulsified oil adjuvant were prepared, except for 1 × 108 TCID50/dose of which only two vaccine were tested. Each vaccine set was evaluated in two independent experiments in guinea pigs and two independent field trials in bovines. Each group included 5 guinea pigs and 5 bovines, except for vaccine with 1 × 106, where 6 animals were added to increase the number of replicates in the concentration considered, under these conditions, the detection limit of the model. Groups of 3–5 animals, vaccinated with placebo and non-vaccinated, were included in each assay as negative controls.
Reference vaccines: Second set of vaccines of known antigen concentration (potency) prepared under industrial conditions used for concordance analysis included water-in-oil emulsions.
Oil commercial vaccines included water-in-oil and double water-oil-water emulsions.
Some vaccines were tested in more than one occasion to evaluate stability through time, generating a higher number of comparison lines.