Table 3.

Concordance between the guinea pig model and bovine, analyzed by ELISA (a) and VN (b).^a.

Guinea pig/Bovine	Unsatisfactory $\overline{x}<1.69$	Satisfactory $1.69\le \overline{x}<2.72$	Very satisfactory $2.72\le \overline{x}$
(a) ELISA; n = 63
Unsatisfactory $\overline{x}<1.93$	36	2	0
Satisfactory $1.93\le \overline{x}<3.02$	0	5	3
Very satisfactory $3.02\le \overline{x}$	0	1	16

Guinea pig/Bovine	Unsatisfactory $\overline{x}$ < 1. 27	Satisfactory 1.27 ≤ $\overline{x}$ < 1.96	Very satisfactory 1.96 ≤ $\overline{x}$
(b) VN; n = 63
Unsatisfactory $\overline{x}<1.31$	37	0	1
Satisfactory $1.31\le \overline{x}<2.05$	1	4	1
Very satisfactory 2.05 ≤ $\overline{x}$	0	3	16

ELISA Weighted Kappa: 0.894; ASE = 0.041; 95%CI 0.813–0.974; p < 0.0001; almost perfect agreement.

VN Weighted Kappa: 0.876; ASE = 0.050; 95%CI 0.777–0.971; p < 0.0001; almost perfect agreement.

^aThe analysis included the 20 assays corresponding to the calibration vaccines used to estimate the doses response curve; 8 placebos, 23 non-vaccinated groups and 12 assays including vaccines of known potency or reference vaccines.