Table 3.
Results of vaccination-challenge test by CK/CH/LHLJ/04V P40, P70 and P110.
| Groupa | Morbidity (%)b | Mortality (%)b | Antibody responsec |
Virus recoveryd |
||||
|---|---|---|---|---|---|---|---|---|
| 4e | 8 | 12 | 16 | Trachea | Kidney | |||
| P40 | 0/10(0) | 0/10(0) | 10/10(100) | 10/10(100) | 10/10(100) | 10/10(100) | 0/10(0) | 0/10(0) |
| P70 | 0/10(0) | 0/10(0) | 10/10(100) | 10/10(100) | 10/10(100) | 10/10(100) | 1/10(10) | 0/10(0) |
| P110 | 3/10(30) | 2/10(20) | 8/10(80) | 10/10(100) | 10/10(100) | 10/10(100) | 6/10(60) | 5/10(50) |
| Positive control | 10/10(100) | 4/10(40) | 0/10(0) | 5/7(70) | 6/6(100) | 6/6(100) | 10/10(100) | 10/10(100) |
| Negative control | 0/5(0) | 0/5(0) | 0/10(0) | 0/5(0) | 0/5(0) | 0/5(0) | 0/5(0) | 0/5(0) |
a
Twenty chicks in groups P40, P70, P110 and the positive control group, and 10 chicks in the negative control group.
b
The morbidity and mortality were those of P40, P70 and P110 vaccinated chickens after challenge.
c
Number seroconverted/number inoculated.
d
Two procedures were used for virus recovery after challenge. First, lesions in embryos that had been inoculated with individual tissue samples (trachea or kidney) were observed. Second, RT-PCR using oligonucleotide primers N(+) and N(−) on RNA recovered from allantoic fluid of the same eggs was conducted. The results from the two procedures were identical.
e
Days after challenge.