Figure 4.

Integration of multiple signals activates a set of transcription factors that together establish the specialized 3D chromatin landscape of effector lymphocytes. Naive B and T lymphocytes require a combination of three signals (1: antigen-receptor ligation, 2: costimulation, 3: cytokines) for their full activation. These three signals will activate signal-responsive TFs (e.g. NFAT, AP-1, STAT), which will in turn activate lineage-determining TFs (e.g. GATA3, T-bet) that together initiate topological genome remodeling in a concerted manner. The resulting specialized 3D chromatin landscape that arises is tailored to the specific microenvironmental input received by the lymphocyte. Of note, complete topological remodeling by TFs appears to require all three signals. Additional abbreviations: BCR; TCR