Table 1. Bacterial strains and vectors used in this study.
| Bacterial strains and vectors | Description | Source |
|---|---|---|
| Strains | ||
| B. tequilensis BD69 (Nucleotide accession # MF767893) | Wild type, able to degrade cellulose | Isolated from indigenous buffalo dung samples |
| DH10β (High efficiency) | Competent cells | New England Biolabs (USA) |
| E. coli BL21 (DE3) pLysS | Expression host | Promega (USA) |
| Pichia pastoris GS115 | Expression host | Thermo Fisher Scientific (USA) |
| Vectors | ||
| pET-30a(+) | Expression vector | Novagen (Germany) |
| pPIC9K | Expression vector | Thermo Fisher Scientific (USA) |
| pET-Bteqβgluc | Recombinant expression vector, Bteqβgluc gene cloned into the pET-30a(+) vector | Developed in this study |
| pPIC-Bteqβgluc | Recombinant expression vector, Bteqβgluc gene cloned into the pPIC9K vector | Developed in this study |