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. 2020 Mar 30;8:e8792. doi: 10.7717/peerj.8792

Table 1. Bacterial strains and vectors used in this study.

Bacterial strains and vectors Description Source
Strains
B. tequilensis BD69 (Nucleotide accession # MF767893) Wild type, able to degrade cellulose Isolated from indigenous buffalo dung samples
DH10β (High efficiency) Competent cells New England Biolabs (USA)
E. coli BL21 (DE3) pLysS Expression host Promega (USA)
Pichia pastoris GS115 Expression host Thermo Fisher Scientific (USA)
Vectors
pET-30a(+) Expression vector Novagen (Germany)
pPIC9K Expression vector Thermo Fisher Scientific (USA)
pET-Bteqβgluc Recombinant expression vector, Bteqβgluc gene cloned into the pET-30a(+) vector Developed in this study
pPIC-Bteqβgluc Recombinant expression vector, Bteqβgluc gene cloned into the pPIC9K vector Developed in this study