Table 2. Comparison of activity parameters among selected beta glucosidases.
A comparison of physical and kinetic parameters determined for β-glucosidases of natural (N) and cloned (C) form from various microbial species. Units for kcat/Km are as provided for kcat and Km.
| Organism | Form | Optimum Temp & pH | Kinetic parameters | References | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vmax | Km(mM) | kcat(min−1) | kcat/Km | pKa1 | pKa2 | Ea(kJ/mol) | ΔH‡(kJ/mol) | ΔG‡(kJ/mol) | ΔS‡(kJ/mol/K) | ||||
| Bacillus tequelensis BD69- pPIC-Bteqβgluc | C | 50 °C & 5.0 | 1,462.25 U/mg | 7.43 | 454.05 | 61.12 | 4.8 | 6.4 | 44.18 | 41.69 | 57.88 | −54.29 | Present study |
| Bacillus tequelensis BD69- pET-Bteqβgluc | C | 50 °C & 5.0 | 1,445.09 U/mg | 8.43 | 448.72 | 53.24 | 4.8 | 6.4 | 45.29 | 42.82 | 57.85 | −50.46 | Present study |
| Aspergillus niger | N | – | – | 0.24 | 43.15 | 59.71 | – | – | 50 | – | – | – | Javed et al. (2018) |
| Methylococcus capsulatus | C | 70 °C & 6.0 | 0.025 103 U | 0.12 | 3,330.5 | 27,597.0 | – | – | – | – | – | – | Sathe et al. (2017) |
| Thermotoga naphthophila | C | 85 °C & 5.0 | 153 × 103U/mg | 0.45 | 20,238.08 | 2,698,413 | – | – | 27.07 | 24.09 | 46.55 | −62.74 | Akram, Iu & Mukhtar (2018) |
| Thermotoga naphthophila RKU-10 | C | 95 °C & 7.0 | 297 × 10 3U/mg | 1.5 | 25,462.97 | – | – | – | 28.74 | 25.7 | 47.24 | −58.6 | Akram et al. (2016) |
| Aspergillus niger | N | 50 °C & 5.0 | 166 U/mg | 8 | – | – | – | – | – | – | – | – | Narasimha et al. (2016) |
| Bacillus sp. SJ-10 | C | 40 °C & 6.0 | 1.526 U/mg | 4.6 | 13.6 × 105 | 3.0 × 105 | – | – | – | – | – | – | Lee et al. (2015) |
| Thermotoga maritima | C | 80–100 °C & 5.0–7.0 | 238 ± 2.4 U/mg | 0.56 | 3.12 | – | – | – | 36.92 | 33.73 | 127.96 | −246.46 | Mehmood et al. (2014) |
| Bacillus subtilis strain PS | C | 60 °C & 5.0 | 0.94 U | – | 6.92 ×10 −6 | – | – | – | – | – | – | – | Keerti et al. (2014) |
| white rot fungi | N | 60 °C–70 °C & 3.5–5.0 | 0.21 μ g/min (T. versicolor) 9.70 μ g/min (G. frondosa-28) | 0.47 × 10−3(A. auricular-1120)719 × 10−3(L. edodes-7) | – | – | – | – | – | – | – | – | Mfombep, Senwo & Isikhuemhen (2013) |
| Fusarium solani | N | 65 °C & 4.5 | 55.6 U | 1 | – | – | – | – | 53.31 | – | – | – | Bhatti, Batool & Afzal (2013) |
| Bacillus licheniformis | C | 50 °C & 6.0 | 1.26 U/mg | 0.206 | – | – | 5.5 | 7 | 66.31 | 64.04 | – | 48.28 ×10−3 | Zahoor et al. (2011) |
| Bacillus subtilis Natto | C | 45 °C & 6.0 | 1.52 U/mg | – | 0.118 | 3.01 | – | – | – | – | – | – | Kuo & Lee (2008) |
| Melanocarpus sp. MTCC 3922 | N | 60 °C & 6.0 | 43.68 U/mg | 3.3 | 4 × 103 | – | – | – | – | – | – | – | Kaur et al. (2007) |
| Cellulomonas biazotea | N | 52 °C & 7.0 | – | – | – | – | – | – | 88 | 108 | – | 86 ×10−3 | Rajoka et al. (2015) |
| Cellulomonas biazotea NIAB 442 | N | 38 °C & 6.6 | 0.22 U/mg | 4.25 | – | – | – | – | 65 | – | – | – | Siddiqui et al. (1997a) |