Fig. 1.

Overexpression of ORF-6-induced apoptosis in different cell lines. (A), COS-7 and (B) Vero E6 cells were transiently transfected with GFP and HA-Bax, GFP-N1, GFP-ORF-6 and GFP-ORF-7a for 24 h. The nuclei of the cells were stained by Hoechst for 15 min. The number of healthy cells was counted under fluorescence microscopy with no DNA condensation and fragmentation. The percentage of apoptotic cells was calculated by the number of healthy cells over the total number of transfected cells. Experiments were repeated three times. Standard deviations are shown.