Figure 4.

(A) Total retinal thickness measurements of the injection area were equivalent in Abca4^−/− mice that received a sham injection in one eye and dual vector (2E+10 gc) in the contralateral eye 3 and 6 months after injection (n = 6). (B) No significant differences in scotopic a-wave and b-wave (0.1 cd.s/m²) amplitudes were identified between paired injected eyes that received either sham injection or dual vector at 5 months after injection (n = 6, two-way analysis of variance with Sidak's multiple comparisons: scotopic a-wave P = 0.862, scotopic b-wave P = 0.4742). (C) Representative OCT sections through the region of subretinal injection in paired sham (top) and dual vector (bottom) injected eyes. Blue arrows indicate the approximate location of the needle when performing subretinal injection.