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. Author manuscript; available in PMC: 2020 Jul 30.
Published in final edited form as: Biochemistry. 2018 Sep 28;57(41):5996–6002. doi: 10.1021/acs.biochem.8b00835

Figure 5. Calorimetric titration for the binding of peptides to ThcOx.

Figure 5

The top panel shows the injection of peptides (400 μM) into the cell filled with ThcOx at 20 °C, yielding an exothermic binding isotherm. The bottom panel shows the integrated data obtained from the raw data, after subtracting the heat of dilution. Experimental data were fitted by using the one set of sites model from MicroCal Origin. (a) Binding of PatE3′ with ThcOx. (b) Binding of ThcE1 and ThcOx.