| Potential Problem | Possible Reason | Suggested Solution |
|---|---|---|
| Surface expression of the receptor is not detected | There may be an issue with DNA construct or DNA transfection | Confirm the complete sequence of the DNA plasmid and double check its purity and integrity. Optimize the transfection condition by varying the DNA:PEI ratio and cell confluence |
| Endocytosis of the receptor is not detected | Ligand-stimulation may be sub-optimal | Prepare fresh stock of the ligand and confirm the appropriate concentration for stimulation |
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