Extended Data Fig. 3. Cryo-EM single-particle reconstruction of the β₁AR–βarr1‒F_ab30 complex.

a, Representative micrograph (LMB-Krios2, magnification 105,000×, defocus –1.9 µm) of the β₁AR_6P–βarr1–F_ab30 complex collected using a Titan Krios with the GIF Quantum K2 detector. b, Representative 2D class averages of the β₁AR_6P–βarr1–F_ab30 complex determined using approximately 1 million particles after 3D classification. Copies of the final reconstruction are juxtaposed to indicate relative orientations. c, FSC curve of the final reconstruction (black) showing an overall resolution of 3.3 Å using the gold standard FSC of 0.143. The directional 3D-FSC curves calculated from the two half maps are shown in colour⁷². d, Final reconstruction coloured by polypeptides (contour level 0.023). e. Local resolution estimation of the β₁AR_6P–βarr1–F_ab30 map as calculated by Relion.