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. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Nat Immunol. 2019 Oct 7;20(11):1517–1529. doi: 10.1038/s41590-019-0490-2

Fig. 1. Splenomegaly in mice with conditional loss of Ikaros in mature B cells.

Fig. 1

a, Increased spleen size upon conditional Ikzf1 deletion in mature B cells. The weight of the spleen was determined for experimental Cd23-Cre Ikzf1 fl/– (n = 15) and Cd23-Cre Ikzf1 fl/fl (n = 51) mice (collectively referred to as Ikzf1 B–; black) and for control Ikzf1 fl/fl (n = 17), Ikzf1 fl/+ (n = 9), Ikzf1 fl/– (n = 5) and Cd23-Cre Ikzf1 fl/+ (n = 29) mice (collectively referred to as Ikzf1 B+; gray) at the age of 5-10 weeks. b, Age-dependent increase of the spleen size. The weight of the spleen was determined for Ikzf1 B– (black, n = 4-21) and Ikzf1 B+ (gray, n = 6-17) mice at the indicated ages. c, Relative frequencies of different hematopoietic cell types (upper panel) and B cell subsets (lower panel) among total live cells in the spleen of Ikzf1 B– (black) and Ikzf1 B+ (gray) mice (n = 17-29), as determined by flow cytometric analysis. d, Evaluation of Ikzf1 deletion through analysis of Ikaros expression by intracellular staining and flow cytometry of the indicated splenic cell types in Ikzf1 B– (black line) and Ikzf1 B+ (gray) mice. The percentage of Ikaros-deficient cells is indicated. e, Flow cytometric analysis of mature splenic B-2 cell subsets (CD19+CD5–CD138–CD93–) in Ikzf1 B– and Ikzf1 B+ mice. The percentage of cells in each gate is indicated. f, Determination of the cell size and expression of the activation marker MHCII by flow cytometric analysis of splenic FO, CD21loCD23– and CD21–CD23– B cells of the indicated genotypes. Data were obtained in 36 (a,c) and 60 (b) independent experiments, and data in d-f are representative of 20 (d,e,f (size)) and 5 (f (MHCII)) independent experiments. Mice at the age of 5-10 weeks (c-f) were analyzed. Statistical data (a-c) are shown as mean values with SEM and were analyzed by one-way ANOVA (Tukey; a) or by multiple t-tests (unpaired, two-tailed, Holm-Sidac; b,c): *P < 0.03, **P < 0.002, ***P < 0.0002, ****P < 0.0001. See Source Data for exact description of the mouse numbers (n) and P values. Each dot corresponds to one mouse. The different cell types were defined as described in the Online Methods.