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. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Sci Immunol. 2019 Dec 13;4(42):eaav7501. doi: 10.1126/sciimmunol.aav7501

Fig. 4. Prolonged STAT2 activation but no change to dephosphorylation rate. All data are from patient II:3 and control fibroblasts.

Fig. 4

(A) pSTAT2 timecourse (immunoblot, 1000 IU/mL IFNα) and (B) densitometry analysis (n=5 experiments, two-way ANOVA with Sidak’s post-test). (C) Immunofluorescence analysis (1000 IU/mL IFNα, scale bar = 100μm, representative of n=3 experiments) with (D) image analysis of STAT2 nuclear translocation (n=100 cells/condition, ANOVA with Sidak’s post-test). (E) RNA-seq analysis of interferon-regulated genes (n=3 controls) with (F) validation by RT-PCR (n=3, two-way ANOVA with Sidak’s post-test). (G) pSTAT2 decay (immunoblot). 1000 IU/mL IFNα (30 min) followed by extensive washing and treatment with 500nM staurosporine (STAU). Times relative to STAU treatment. (H) No significant differences by densitometry analysis (n=3, t test). Data are presented as mean ± SEM of repeat experiments. *P<0.05, ** <0.01 *** <0.001, **** <0.0001, n.s.= nonsignificant.