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. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Sci Immunol. 2019 Dec 13;4(42):eaav7501. doi: 10.1126/sciimmunol.aav7501

Fig. 6. STAT2R148W fails to support desensitization through its impaired interaction with USP18.

Fig. 6

(A) Desensitization assay (immunoblot, fibroblasts) with (B) pSTAT densitometry analysis (pSTAT/tubulin, ratio to unprimed, n=4, ANOVA with Sidak’s post-test). (C) Schematic of USP18 mechanism of action and proposed model of STAT2R148W pathomechanism. (D) Modelling of exposed WT (R148)/mutant (W148) residue, demonstrating charge-change (blue = positive, red = negative) and possible steric restriction. (E) Co-immunoprecipitation of USP18 by STAT2 in U6A cells expressing STAT2WT or STAT2R148W with (F) densitometry analysis (USP18/STAT2, ratio to WT, one-sample t test). Data are mean ± SEM (** P<0.01, **** <0.0001).