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. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Sci Immunol. 2019 Dec 13;4(42):eaav7501. doi: 10.1126/sciimmunol.aav7501

Fig. 7. USP18 insensitivity in cells bearing STAT2R148W.

Fig. 7

All data are from patient II:3 and control fibroblasts. (A) STAT phosphorylation in USP18 and vector expressing fibroblasts (immunoblot) with (B) pSTAT densitometry analysis (pSTAT/tubulin, ratio to unprimed, n=3, ANOVA with Sidak’s post-test). (C) Immunofluorescence analysis of STAT2 nuclear translocation (1000 IU/ml IFNα, scale bar = 100μm, representative of n=3 experiments) with (D) image analysis (n=100 cells/condition, ANOVA with Sidak’s post-test). (E) Timecourse of STAT phosphorylation upon IFNα stimulation (1000 IU/mL for 0, 1, 6 and 24 h) of cells transduced with USP18 shRNA or non-targeting (NT) shRNA with (F) densitometry analysis of pSTAT2 (n=3, t test). Data are mean ± SEM (** P<0.01, *** <0.001, **** <0.0001, ns = nonsignificant).