Figure 3. Stat1^−/− mice recapitulate the phenotype of coexisting MPN and B-cell transformation.

(A) BM of Statl ^−/− mice with 6 weeks of age (n = 3), 4 months of age (n = 3), and Statl ^−/− MPN⁺ mice (n = 6) was analyzed for D-J rearrangement of the IgH gene. Pc, polyclonal B cells (derived from splenocytes of a wt mouse). Asterisks denote samples of those mice, whose B-cell clonality was followed in subsequent transplantations (outlined in supplemental Figure 5C). (B) BMs or spleens of MPN⁺ Statl ^−/− mice were fractionated into LSK (Lin⁻Sca-1⁺c-Kit⁺), progenitors (Lin⁻c-Kit⁺ Sca-1), CD3⁺, Mad⁺, Gr1⁺Mac1⁺, CD19⁺, and Lin⁺ cells and intravenous injected into Rag2^−/− γc^/ mice. Table shows incidence of B-cell disease in recipient mice that had received individual populations or whole BM. (C) Cytospins of Statl ^−/− B-cell lines: #503, #1762, #6500 and #7473. Original magnification: 40X. Scale bar, 10 μm.