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. Author manuscript; available in PMC: 2020 Aug 11.
Published in final edited form as: Nat Protoc. 2019 Jul 22;14(8):2279–2317. doi: 10.1038/s41596-019-0185-z

Figure 6. Data analysis of eukaryotic selective ribosome profiling data.

Figure 6

Initial read processing trims adaptors, removes low quality and noncoding reads, and aligns the filtered reads to the genome of interest. Python scripts to perform subsequent analyses including ribosome A-site assignment, RP quality assessment and Basic SeRP analysis are explained in detail in the text.