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. Author manuscript; available in PMC: 2020 Aug 11.
Published in final edited form as: Nat Protoc. 2019 Jul 22;14(8):2279–2317. doi: 10.1038/s41596-019-0185-z
SCANNING SETTINGS
Scanning mode: UV OD WITH SINGLE
FLUORESCENCE SCAN
Channel A (LED1) Wavelength: 260 nm
Channel B (LED2) Fluor: EGFP
Excitation (LED2) Wavelength: 474 nm
Excitation filter A: 474/50 nm
Emission filter A: 535/50 nm
Sensitivity: 0
Integration time: 320 ms
Averaging: 1 samples
LED1 (260 nm) On Time: 64 ms
PD2 (474 nm) On Time: 320 ms
Sample A Zero: 792807
Source A Zero: 325159.1
Fluorescence Zero: 50