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. Author manuscript; available in PMC: 2020 Aug 29.
Published in final edited form as: Gastroenterology. 2010 Dec 11;140(3):947–56. doi: 10.1053/j.gastro.2010.12.005

Figure 4. Signature of BCR activation in PP GCs.

Figure 4

A. Photomicrograph illustrating Lyn translocation to the cell membrane, in keeping with Lyn activation upon BCR engagement by antigen (and inset higher magnification). B. Relative quantitation of Lyn gene expression (4 individuals studied) by isolated GC (IgD-CD10+), mantle zone (IgD+CD10-) and marginal zone (IgD-CD10-) cells. Data is represented as relative quantitation normalized to average GC=1 (red dotted line). B cells isolated from PPs show no significant difference in Lyn mRNA expression in the three populations. C. IHC on PP GC showing low protein expression in the PP GCs immunostained with anti-CD22 monoclonal antibody, as compared with the mantle or marginal zones (and inset lower magnification). D. Accordingly, significant down-regulation of CD22 transcription in PP GCs was observed (p=0.03 GC vs. mantle zone). (Original magnification 200x in A and C and 100x in inset). E and F. Isolated PP GC cells show increased transcription of the BCR regulated genes, Jun and Fos.