Figure 1. TLR7 and TLR8 Are Functional TLRs in BLaER1 Monocytes.
(A and B) BLaER1 monocytes of indicated genotypes were stimulated with (A) TL8-506, R848, and LPS or were (B) unstimulated or stimulated with pR and RNA40S. After 14 h, IL-6 release was measured. (C) TLR8 expression in BLaER1 cells of indicated genotypes. (D) Control and TLR8-deficient BLaER1 monocytes were stimulated with increasing amounts of RNA40S. After 14 h, IL-6 was measured. Data are depicted as mean + SEM of three independent experiments (A, B, and D) or one of three representative blots (C). Statistics indicate significance by two-way ANOVA: ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05; ns, not significant. See also Figure S1.