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. Author manuscript; available in PMC: 2020 Nov 27.
Published in final edited form as: Cell. 2019 Nov 27;179(6):1264–1275.e13. doi: 10.1016/j.cell.2019.11.001

Figure 2. RNase-T2-Deficient Cells Fail to Respond to RNA Oligonucleotides.

Figure 2

(A) Venn diagram of proteins that are, according to their Gene Ontology (GO) terms, annotated as “lysosomal,” “extracellular space,” or “ribonuclease activity” (left). A heatmap shows RNA expression levels of several RNases and their GO-term designation in the indicated cell types (middle and right). (B) IL-6 production in BLaER1 Controls and RNASET2 −/− cells stimulated with RNA40S, TL8-506, R848, and LPS for 14 h. (C) RNASET2 −/− cells were reconstituted with doxycycline-inducible RNase T2 (++, 1 μg/mL; +, 0.5 μg/mL). An inducible mScarlet construct was used as a control. (D) The immunoblot corresponding to the reconstitution experiment is shown. The asterisk indicates unspecific bands. Data are depicted as mean + SEM of three independent experiments or one of three representative blots. Statistics indicate significance by two-way ANOVA: ***p ≤ 0.001; **p ≤ 0.01; *p ≤ 0.05; ns, not significant. See also Figure S2.