. Author manuscript; available in PMC: 2020 Nov 27.

Published in final edited form as: Cell. 2019 Nov 27;179(6):1264–1275.e13. doi: 10.1016/j.cell.2019.11.001

Key Resources Table.

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Direct-Blot HRP anti-FLAG tag	BioLegend	Cat#637311
Anti-RNase T2	Sigma-Aldrich	Cat#HPA029013
Anti-rabbit IgG HRP linked	Cell Signaling Technology	Cat#7074
Anti-TLR8 (D3Z6J)	Cell Signaling Technology	Cat#11886S
Bacterial Strains
S. aureus subsp. aureus	ATCC	ATCC 6538
Chemicals
Agilent RNA 6000 Nano Kit	Agilent	Cat#5067-1511
Ammonium persulfate	Sigma-Aldrich	Cat#A3678
Blood agar plate	OXOID	Cat#PB5039A
Doxycycline hyclate	Sigma-Aldrich	Cat#D9891
EDTA (0.5M)	Thermo Fisher	Cat#15575020
GeneJuice^® Transfection Reagent	Merck Chemicals GmbH	Cat#70967
Gentamicin	Thermo Fisher	Cat#15750060
HisTrap™	GE Healthcare	Cat#17-5247-01
ISOGRO^®-¹⁵N Powder-Growth Medium	Sigma-Aldrich	Cat#606871
Lipofectamine 2000 Transfection Reagent	Thermo Fisher Scientific	Cat#11668019
LPS-EB Ultrapure	InvivoGen	Cat#tlrl-3pelps
nitrocellulose membrane (0.45μm)	GE Healthcare	Cat#10600002
Pam3CSK4	Invivogen	Cat#tlrl-pms
Penecilin-Streptomycin	Life Technologies	Cat#15140122
Phenol/Chloroform/Isoamylalkohol	Roth	Cat#A156.3
Phorbol 12-myristate 13-acetate (PMA)	Enzo Life Sciences	Cat#BML-PE160-0005
poly-L-arginine	Sigma-Aldrich	Cat#P7762
Recombinant Human IL-3	MPI of Biochemistry, Munich	N/A
Recombinant Human INF-γ	PeproTech	Cat#300-02
Recombinant Human M-CSF	MPI of Biochemistry, Munich	N/A
R848 SequaGel Concentrate	InvivoGen national diagnostics	Cat# tlrl-r848 Cat#EC8301L
SequaGel Buffer	national diagnostics	Cat#EC835200ML
SequaGel Diluent	national diagnostics	Cat# EC8401L
Superdex-200 16/600	GE Healthcare	Cat#28989335
SYBR Gold Nucleic Acid Gel Stain	Thermo Fisher Scientific	Cat#S11494
TL8-506	InvivoGen	Cat# tlrl-tl8506
TEMED	Roth	Cat#2367.3
UltraPure 0.5M EDTA, pH 8.0	Thermo Fisher Scientific	Cat#15575020
ß-Estradiol	Sigma-Aldrich	Cat#E8875-250MG
Critical Commercial Assays
Human IL-6 ELISA Set	BD Biosciences	Cat#555220
MiSeq Reagent Kit v2, 300 Cycles	Illumina	Cat#MS-102-2002
Deposited Data
BLaER1 RNA Seq data	This paper	GEO: GSE138913
Experimental Models: Cell Lines
BLaER1 human b-cell to monocyte trans-differentiation cell line	(Rapino et al., 2013)	N/A
HEK293T	(Gaidt et al., 2017)	N/A
THP-1	DSMZ	Cat#ACC 16
Oligonucleotides
RNA40S (rGrCrCrCrGrUrCrUrGrUrUrGrUrGrU* rGrArCrUrC)	Miltenyi	130-104-429
RNA40 (rGrCrCrCrGrUrCrUrGrUrUrGrUrGrUrGrArCrUrC)	IDT	N/A
P20 (rUrUrGrArArGrGrArCrArUrGrUrCrCrUrUrCrArA)	IDT	N/A
P20-5M (rUrGrUrCrCrUrGrArCrArUrGrUrCrCrUrUrCrArA)	IDT	N/A
dNdNdNdNdNdNUUGUdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUGAdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUGGdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUGCdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUGdNdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUAUdNdNdNdNdNdNdN*dN	IDT	N/A
dNdNdNdNdNdNUUAAdNdNdNdNdNdNdN*dN	IDT	N/A
rArArArGrGrArArA	IDT	N/A
rArArArGrArArArA	IDT	N/A
rArArArGrUrArArA	IDT	N/A
rArArArGrCrArArA	IDT	N/A
rArArArArGrArArA	IDT	N/A
rArArArArArArArA	IDT	N/A
rArArArArUrArArA	IDT	N/A
rArArArArCrArArA	IDT	N/A
rArArArUrGrArArA	IDT	N/A
rArArArUrArArArA	IDT	N/A
rArArArUrUrArArA	IDT	N/A
rArArArUrCrArArA	IDT	N/A
rArArArCrGrArArA	IDT	N/A
rArArArCrArArArA	IDT	N/A
rArArArCrUrArArA	IDT	N/A
rArArArCrCrArArA	IDT	N/A
rArCrArCrArCrArCrArCrArCrArCrUrUrGrUrC*rU	IDT	N/A
dAdCdAdCdAdCdAdCdAdCdAdCdAdCdAdC	IDT	N/A
dAdCdAdCdAdCdAdCdAdCdAdCdAdCrUrUrGrUrCrU	IDT	N/A
dAdCdAdCdAdCdAdCdAdCdAdCdAdCrUrU*rG	IDT	N/A
dAdCdAdCdAdCdAdCdAdCdAdCdAdCrUrU*rG-3‘-phosphate	IDT	N/A
dAdCdAdCdAdCdAdCdAdCdAdCdAdCrUrU	IDT	N/A
Recombinant DNA
pcDNA3.1_RNase T2_PreScission_6xHis	This study	N/A
pLI_hu_RNase T2_FLAG_Puro	This study	N/A
pLI_mScarlet _Puro	This study	N/A
CMV-mCherry-Cas9	(Schmid-Burgk et al., 2014)	N/A
pLK0.1-gRNA-CMV-GFP	(Schmid-Burgk et al., 2014)	N/A
pCAS9-mCherry -gRNA	(Schmid-Burgk et al., 2016)	N/A
Software and Algorithms
GraphPad Prism 8	GraphPad	N/A
Outknocker	(Schmid-Burgk et al., 2014)	N/A