Skip to main content
. Author manuscript; available in PMC: 2021 Feb 21.
Published in final edited form as: ACS Synth Biol. 2020 Jul 27;9(8):2023–2038. doi: 10.1021/acssynbio.0c00111

Figure 1. Schematic overview of the GntR-dependent mechanism of counter-silencing.

Figure 1

Synthetic counter-silencer constructs are based on phage promoters targeted by the xenogeneic silencer CgpS (red ovals). The 15 bp short operator sequence (black arrows) of the regulator of gluconate catabolism GntR (blue ovals)28 was inserted within the silenced promoter regions. In the absence of the effector molecule gluconate, binding of GntR to its operator sequence will interfere with the silencer-DNA complex leading to transcription initiation. Adapted from18.