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Shift in frequency (blue) and dissipation (red) during the formation of the S-layer supported lipid membrane and the subsequent treatment with subtilisin A. (A) Injection of the SbpA solution; (B) activation of SbpA; (C) addition of anchor lipids (DMPE); (D) rinsing with 70% ethanol; (E) addition of membraneforming lipids (DOPC) in ethanol; (F) rapid solvent exchange from organic to aqueous phase to form a membrane; (G) addition of subtilisin A; and (H) rinsing with HEPES buffer.
