Figure 6.

Gel images for the multiplex ITS xenomonitoring (MIX) PCR amplicons for 94 non-patent Bulinus globosus collected from Wambaa, Pemba, United Republic of Tanzania. The text under each amplicon denotes the outcome of the Schistosoma sp. targeted sequencing where relevant (i.e., presence of 538 bp amplicon), which resulted in either S. haematobium (S.h.) or sequencing failure (F). The presence of a trematode band without the presence of the Schistosoma band indicated a non-Schistosoma trematode infection (Tr.). Other non-specific bands, in this case, larger bands (NA), were also observed in these snail populations, which did not amplify with the secondary SIX PCR. x = sample failure with no control amplicon. Arrows highlight the presence of the ~1000 bp trematode band when present (n = 8). B. globosus with a patent S. haematobium infection (Cham10.1 see [6]) was run as a positive control (+ve) and also represented the amplicon profile obtained for the seven patent B. globosus snails (five and two with S. bovis and S. haematobium infections, respectively (see Section 2.4). -ve = the non-template negative control. L1—HyperLadder IV (Bioline, London, UK).