. Author manuscript; available in PMC: 2020 Oct 1.

Published in final edited form as: Mol Pharmacol. 2020 Sep 1;98(3):250–266. doi: 10.1124/molpharm.120.000003

Table 1.

Complex formation between dopamine D₁-receptor and wild-type adenosine A₁-receptor or the mutant A₁R-G279S^7.44 BRET₅₀ refers to the amount of plasmid encoding wild-type or mutant adenosine A₁-receptor giving half-maximum bioluminescence resonance energy transfer; maximum BRET is the response estimated at saturation. The values (means ± S.D.) were calculated by fitting the data from three independent experiments (summarized in Fig. 4) to the equation of a rectangular hyperbola.

Incubation	A₁-receptor variant	BRET₅₀	Maximum BRET signal
		μg A₁R plasmid	milli BRET
Control	Wild-type A₁R	0.6 ± 0.1	177 ± 9
	A₁R-G279S^7.44	0.5 ± 0.1	160 ± 30
CPA (10 μM)	Wild-type A₁R	0.9 ± 0.2	218 ± 64
	A₁R-G279S^7.44	0.9 ± 0.2	202 ± 37
Dopamine (10 μM)	Wild-type A₁R	0.7 ± 0.1	207 ± 27
	A₁R-G279S^7.44	0.8 ± 0.1	190 ± 28
CPA and dopamine (10 μM each)	Wild-type A₁R	1.1 ± 0.3	226 ± 82
	A₁R-G279S^7.44	1.4 ± 0.6	229 ± 23