Figure S2.

Behavior across All Mice during Conditioning and Inference Test, Related to Figures 1 and 2

In mice: (A-I) Data Analysis with Bootstrap-coupled ESTimation (DABEST) plots (Ho et al., 2019) used to visualize the effect size of behavioral measures of reward seeking bias. Raw data points are shown for set 1 and set 2 in orange and green respectively, with mean ± SEM shown by black-dot and black-ticks respectively. The effect size for the difference between set 1 and 2 (i.e., reward seeking bias) is shown as a sampling-error distribution, computed from 10,000 bias-corrected bootstrapped resamples (Efron, 2000): black-dot, mean; black-ticks, 95% confidence interval; filled-curve, sampling-error distribution; yellow, laser On; gray, laser Off. (A-H) Across recording days in all mice. (A-C) During visual cues (Yn) in conditioning, greater reward-seeking bias was observed for ‘set 1’ relative to ‘set 2’, with reward-seeking bias defined as the percentage time spent in the outcome area (B, p < 0.001), or defined as time spent licking in anticipation of an outcome (C, p < 0.001). Each data point shows the average reward-seeking bias of a single mouse on a given day. (D-F) After visual cues in conditioning, during the outcome period (Zn), greater reward-seeking bias was observed for ‘set 1’ relative to ‘set 2’, with reward-seeking bias defined as the percentage of time spent in the outcome area (E, p < 0.001), or defined as time spent licking the outcome (F, p < 0.001). Each data point shows the average reward-seeking bias of a single mouse on a given day. (G-H) Following auditory cues (Xn) in the inference test, greater reward-seeking bias was observed for ‘set 1’ relative to ‘set 2’, with reward-seeking bias defined as the percentage time spent in the outcome area (p < 0.001; with one data point at coordinates [1,2;0.56,0.35] off the display). (I) In ArchT-GFP mice, dCA1 light delivery during auditory cues (Xn) in the inference test impaired reward-seeking bias observed for ‘set 1’ relative to ‘set 2’ (Figure 2I). This effect was further observed using alternative measures of reward seeking bias shown here, where reward seeking bias is defined as the percentage of trials with visit to outcome area following the auditory cue (laser Off p < 0.001; laser On p = 0.105; laser Off – laser On: t₅₄ = 3.86, p < 0.001).