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. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Chem Commun (Camb). 2020 May 11;56(47):6420–6423. doi: 10.1039/d0cc02321c

Fig. 2.

Fig. 2

Chemical synthesis of TFF1 and TFF1 homodimer. (A) Analytical C18-RP-HPLC trace of NCL at 0 h. NCL occurred after the hydrazide fragment was converted into a thioester peptide through in situ activation and thiolysis. (B) Analytical C18-RP-HPLC and MS traces of NCL at 24 h. (C) Analytical C3-RP-HPLC and MS traces of folded TFF1. Reduced full-length TFF1 was oxidised in 0.1 M NH4HCO3, pH 8.5 for 48 h. (D) Analytical C3-RP-HPLC and MS traces of folded TFF1 homodimer. TFF1 was dimerised over 48 h in water.