Figure 5.
Development of liver-like tissue analogues in Matrigel (MG) and gelPEG-based hydrogels. A functional read-out of protein levels and enzyme activities was performed after 9 d culture of liver organoids. H&E staining of liver organoids in a) Matrigel, b) gelPEG, c) gelPEG+LN111, and d) gelPEG+LN521. e) Albumin protein levels in cultured organoid cell lysates. f,g) ALAT an ASAT enzyme activities of liver organoid cell lysates. h,i) LDH and GLDH enzyme levels of liver organoid cell lysates. Data are depicted as mean + SD; N = 3, n = 3.