Figure 1. OXPHOS subunit immunohistochemical and histochemical analysis of human colorectal adenomas and adenocarcinomas.
a: Immunohistochemistry was performed on 26 adenocarcinoma samples and patient matched normal mucosa, and 9 colonic adenoma samples. Representative images showing OXPHOS subunit expression in normal mucosa (CSC024), an adenoma (AD07), and an adenocarcinoma (CSC024). b: Cytochrome c oxidase and succinate dehydrogenase histochemistry was performed on the same samples as in panel a. Representative images of normal colonic mucosa and adenocarcinoma from CRC009 are shown in b. c: Quantification of the mean percentage of normal crypts per subject with defects in the specified OXPHOS subunits (each dot represents the mean percentage of OXPHOS deficient crypts in each subject, n=26 subjects, error bars are s.e.m.), and the percentage of adenomas (n=9) and adenocarcinomas (n=26) analysed with defects in the specified OXPHOS subunits. Scale bars a- 20µm, b 50µm.