Extended Data Fig. 6. Quantification of major mass isotopomers following growth of adenoma organods in ¹³C₆-glucose and adenoma organoid growth in to the presence of metformin.

a: Quantification of major mass isotopomers following growth in the presence of ¹³C₆-glucose for 24 hours. ¹³C labelling is shown as M+6 (glucose) and M+0 denotes no labelling. No significant differences were found between organoids from Apc^fl/fl mice compared with PolgA^mut/mut;Apc^fl/fl mice by one-tailed unpaired t-test. n=3 mice per group with 3 technical replicates performed per mouse. Error bars show s.e.m. b: A shared group estimation plot comparing the effect of metformin on the volume of individual adenoma organoids generated from Apc^fl/fl mice (n=3) on days 1 and 5 post seeding. Volume data are normalised to day 1. On day 1 the numbers of organoids measured were: 0µM: n=739, 100µM: n=796, 250µM: n=711, 500µM: n=652. On day 5 the numbers of organoids measured were: 0µM: n=1060, 100µM: n=1515, 250µM: n=1088, 500µM: n=1431. Bootstrap estimation of group mean differences (circle) and 95% confidence intervals (vertical bars) are plotted as a sampling distribution.