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. Author manuscript; available in PMC: 2020 Oct 16.
Published in final edited form as: Biofabrication. 2020 Feb 19;12(2):025014. doi: 10.1088/1758-5090/ab69d9

Figure 5.

Figure 5

In vitro evaluation of bioactivity of the PCaP scaffold. (A) The effect of possible harmful release from composite CaP-based material contained different polymeric carriers on the number of viable cells, (B) the potential of osteogenic differentiation of equine MSCs was investigated through ALP activity, (C) cell proliferation on the C-PCaP filament after cultivation for 7 and 14 days (nucleus (dapi: blue) and F-actin (phalloidin: green)), and (D) cell differentiation on the C-PCaP filament toward an osteogenic lineage after cultivation for 7 and 14 days in an osteogenic medium (nucleus (dapi: blue) and osteonectin protein (osteonectin: red)) (scale bar = 100 μm.).