Mechanical properties of the osteochondral unit. (A) Osteochondral unit (scale bar = 1mm.), (B) representative stress-displacement curves from interfacial shear stress assessment at the interface between the chondral and bone compartment, (C) representative stress-strain curves from compression assessment of chondral compartment, (D) mechanical testing (interfacial shear stress: left, and compressive modulus (right)), (E) interfacial shear stress of an engineered osteochondral unit showing alterations due to differences in either interfacial architecture or compositions (GelMA on ceramic (unmodified surface; red), GelMA on ceramic (modified surface; bright green), microfibre reinforced GelMA on ceramic (non-anchor fibre; pink), microfibre reinforced GelMA on ceramic (anchor fibre; blue) and monolithic GelMA hydrogel (mean (grey dotted line) ± SD (grey filled area))), (F) compressive modulus of chondral compartment showing alterations due to difference in composition (GelMA alone (grey), GelMA over fiat interfacial surface of PCaP (red), microfibre reinforced GelMA alone (dark green), microfibre reinforced GelMA on ceramic (non-anchor fibre; pink), microfibre-reinforced GelMA on ceramic (anchor fibre; blue)), (G) SEM micrographs of cross sections of an osteochondral unit revealing embedded microfibres within non-macro porous layer of the bone compartment of newly fabricated structure and (H) after interfacial shear stress assessment.