LjCCD4 and LjCCD1b catalyzed cleavage of β-carotene, lutein, and 10'-apo-β-carotenal. The 9,10 (9',10') double bonds cleavage sites for LjCCD1b are showed with a blue diagonal line (/) through the carotenoid backbone. The red diagonal line (/) through the carotenoid backbone represents the 9,10 (or 9',10') double bonds cleavage sites for LjCCD4.